BIO-POLY

Welcome to BIO-POLY

INFORMATIVE WEBSITE ON BIOFLAVONOIDS and POLYPHENOLS

BIOFLAVONOIDS and POLYPHENOLS

WHAT IS BIO-POLY

This site is dedicated to give information on blends of BIOFLAVONOIDS and POLYPHENOLS (BIO-POLY) countain in BIOSECUR LAB's products.


BIO-POLY are coming from selected:

     Citrus fruits species: 

          - Reticulata (tangerine), 

          - Reticulata (mandarine); 

          - Sinensis (sweet orange); 

          - Aurantium (bitter orange) 

     Plant Extracts: 

          - Hibiscus Sabdariffa extract


BIOSECUR Lab selected a mixture of POLYPHENOLS and BIOFLAVONOIDS in the purpose to formulate natural food preservatives to eliminate pathogenic and spoilage microorganisms and to ensure food safety.


No more chemical preservatives agents in your food products.


Our scientific researches can help you to find which one of BIOSECUR products that will be suitable to use on your food matrix.

WHAT IS BIO-POLY

This site is dedicated to give information on blends of BIOFLAVONOIDS and POLYPHENOLS (BIO-POLY) countain in BIOSECUR LAB's products.


BIO-POLY are coming from selected:

     Citrus fruits species: 

          - Reticulata (tangerine), 

          - Reticulata (mandarine); 

          - Sinensis (sweet orange); 

          - Aurantium (bitter orange) 

     Plant Extracts: 

          - Hibiscus Sabdariffa extract


BIOSECUR Lab selected a mixture of POLYPHENOLS and BIOFLAVONOIDS in the purpose to formulate natural food preservatives to eliminate pathogenic and spoilage microorganisms and to ensure food safety.


No more chemical preservatives agents in your food products.


Our scientific researches can help you to find which one of BIOSECUR products that will be suitable to use on your food matrix.

ACTION MODE

BIOSECUR Lab use a mixture of BIO-POLY carefully selected to ensure the antimicrobial properties in their products.


According to chemicals analysis BIOSECUR products are rich in BIO-POLY. As an example of these compounds we can found flavanone (naringenin, naringin, neoeriocitrin, neohesperedin and hesperidin), flavonol (kaempferol, quercetin and rutin) etc.


Possible mode of action of BIO-POLY:

1. Increase of the membrane permeability 

2. Damage of the bacterial cell wall 

3. Decrease of the internal bacterial pH 

4. Dismantle of bacterial structures 

5. Destruction of the bacterial DNA

6.Inhibit the communication between bacteria 

7. Reduce up to destroy the biofilm formation 

8. Affect the bacterial mobility 

9. Reduce the bacterial energy (ATP)production


ACTION MODE

BIOSECUR Lab use a mixture of BIO-POLY carefully selected to ensure the antimicrobial properties in their products.


According to chemicals analysis BIOSECUR products are rich in BIO-POLY. As an example of these compounds we can found flavanone (naringenin, naringin, neoeriocitrin, neohesperedin and hesperidin), flavonol (kaempferol, quercetin and rutin) etc.


Possible mode of action of BIO-POLY:

1. Increase of the membrane permeability 

2. Damage of the bacterial cell wall 

3. Decrease of the internal bacterial pH 

4. Dismantle of bacterial structures 

5. Destruction of the bacterial DNA

6.Inhibit the communication between bacteria 

7. Reduce up to destroy the biofilm formation 

8. Affect the bacterial mobility 

9. Reduce the bacterial energy (ATP)production


RESEARCH

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Polyphenols

Polyphenols are compounds with more than one phenol group. They are found ubiquitously in nature. According to Petti and Scully (2009) the function of these plant molecules is to protect the plants against pathogenic microorganisms including predatory insects.  

The most important Polyphenols classes are phenolic acids, which include polymeric structures, such as hydrolyzable tannins, lignans, stilbenes, and flavonoids. Flavonoids include flavonols (example:quercetin and kaempferol), flavones, isoflavones, flavanones, anthocyanidins, flavanols (catechins—monomers and proanthocyanidins—polymers, known as condensed tannins)

Havsteen (2002); Dabbah et al. (1970) found that in vitro, polyphenols have been shown to interfere with Candida albicans in several ways.

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Bioflavonoids and their antimicrobial properties

Flavonoids are hydroxylated phenolic substance known to be synthesized by plants in response to microbial infection and have been found in vitro to be effective antimicrobial substances against a wide array of microorganisms. Marjorie, (1999) reported that their activity is probably due to their ability to complex with extracellular and soluble proteins and to complex with bacterial cell walls. 


According to many scientific studies the bioflavonoids and polyphenols extracted from citrus fruits allowed a high antimicrobial properties against foodborne pathogens such as Gram positive or negative bacteria and yeasts and molds. It's important to note that BIO-POLY exhibited a significant antimicrobial effect as reported by many researches.


Flavonoids such as quercetin, apigenin, kaempferol, galangin and genistein were

reported to possess potent antibacterial activity (Basile et al., 1999; Friedman, 2007; Ohemeng et al., 1993; Rauha et al., 2000).


Geoghegan et al. (2010) found that Quercetin possesses significant antimicrobial properties on periodontal pathogenes in vitro. Indeed the minimum inhibitory concentration were 1000 and 125 ppm against Actinobacillus Actinomycetemcomitans and Porphyromonas gingivalis. According to Cushnie and Lamb (2005) Quercetin have several mode of action such as inhibition of nucleic acid synthesis, inhibition of energy metabolism and inhibition of cytoplasmic membrane function.


Anti-biofilm and anti-communication effect of flavonoids

Vikram et al. (2010) reported that:

Naringenin, kaempferol, quercetin and apigenin interfere on the cell-cell communication system.

Quercetin and kaempferol effect significantly the growth of Vibrio harveyi and E. coli O157:H7 at 100 µg/ ml and induce the Inhibition of biofilm formation in Vibrio harveyi and Escherichia coli O157:H7

Quercetin, naringenin and sinensetin allowed a strong inhibition of biofilm formation.


Vikram et al. (2010) found that naringenin attenuated Salmonella Typhimurium virulence and cell motility.


Hesperidin and Hesperetin antimicrobial activity

According to Iranshahi et al. (2015) polyphenols play an important role in the resistance against various microbial pathogens.

Hesperidin (Hsd), hesperetin (Hst), are two flavonoids from citrus species that exhibit antimicrobial properties.

As an example Hsd and ten other flavonoids isolated from citrus species showed a range of inhibitory effects on food fungal contaminants, including Aspergillus parasiticus, Aspergillus flavus, Fusarium semitectum and Penicillium expansum.


Krolicki and Lamer-Zarawska, (1984) reported that antifungal activity of hesperidin was observed at a dose ranging from 1 to 10 µg, against Botrytis cinerea, Trichoderma glaucum and Aspergillus fumigatus. 


In a study involving the investigation of anti-Helicobacter pylori (HP) activity, in vitro, of some flavonoids and their metabolites, hesperetin and other flavonoids were found to inhibit the growth of HP (Bae et al., 1999).


Antimicrobial effect of Hibiscus extract

The antibacterial activity of Hibiscus sabdariffa extracts can be attributed to the action of the phytochemical compounds it contains (Babayi et al., 2004).


Olaleye (2007) reported that aqueous-methanolic extract of Hibiscus sabdariffa calyces have been found to exhibit antibacterial activities against Staphylococcus aureus, Bacillus stearothemophilus, Micrococcus luteus, Serratia mascences, Clostridium sporogenes, Escherichia coli, Klebsiella pneumonae, Bacillus cereus and Pseudomonas fluorescence.


Mounnissamy et al. (2002) reported that the results of antibacterial activity of gossypetin isolated from Hibiscus sabdariffa revealed that the activity may be due to polyphenolic nature of the flavonoid gossypetin . In addition according to Walsh et al. (2003) the mechanism of action may be by inhibition of various cellular processes, followed by an increase in plasma membrane permeability and finally ion leakage from the bacterial cells. 


Liu et al. (2005) reported that the aqueous extract of Hibiscus Sabdariffa could inhibit several nosocomial infectious bacteria such as methicillin-resistant S. aureus and Klebsiella pneumonia.


On the other hand, the protocatechuic acid, a polyphenol which is containing a 3,4-dihydroxy substructure, is a compound that naturally occurs in roselle calyx. Several in vitro studies have indicated that this compound can inhibit the growth of E. coli and fungi (Fernandez et al., 1996; Aziz et al., 1998).




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References:

A. Vikram, Palmy R. Jesudhasan, G.K. Jayaprakasha, Suresh D. Pillai, Arul Jayaraman, Bhimanagouda S. Patil (2011). Citrus flavonoid represses Salmonella pathogenicity island 1 and motility in S. Typhimurium LT2. International Journal of Food Microbiology; 145:28–36.

http://www.sciencedirect.com/science/article/pii/S0168160510006264


A. Vikram, G.K. Jayaprakasha, P.R. Jesudhasan, S.D. Pillai and B.S. Patil (2010). Suppression of bacterial cell–cell signalling, biofilm formation and type III secretion system by citrus flavonoids Journal of Applied Microbiology, 109(2):515-27. http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2010.04677.x/full


Aziz NH, Farag SE, Mousa LA (1998). Comparative antibacterial and antifungal effects of some phenolic compounds. Microbios 93:43-54.

http://europepmc.org/abstract/med/9670554


Bae EA, Han MJ, Kim DH. (1999). In vitro anti-Helicobacter activity of some flavonoids and their metabolites, Planta Med 65: 442-443.

https://www.ncbi.nlm.nih.gov/pubmed/10454900


Basile, A., Giordano, S., López-Sáez, J.A., Cobianchi, R.C., (1999). Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry 52, 1479–1482.

https://www.ncbi.nlm.nih.gov/pubmed/10647220


Cushnie TPT, Lamb AJ. 2005. Antimicrobial effects of Flavonoids. Int J Antimicrob Agents 26: 343–356.

http://www.sciencedirect.com/science/article/pii/S0924857905002554


Dabbah R, Edwards VM, Moats WA (1970). Antimicrobial action of some citrus fruit oils on

selected food-borne bacteria. Appl Microbiol; 19: 27-31.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC376603/


Fernandez MA, Garcia MD, Saenz MT (1996). Antibacterial activity of the phenolic acids fractions of Scrophularia frutescens and Scrophularia sambucifolia. J. Ethnopharmacol. 26:11-14.

http://www.sciencedirect.com/science/article/pii/0378874196014195


Friedman, M., (2007) Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas. Mol. Nutr. Food Res. 51, 116–134.

https://www.ncbi.nlm.nih.gov/pubmed/17195249


Geoghegan F., Wong R.W.K. and Rabie A.B.M. (2010). Inhibitory effect of Quercetin on Periodontal Pathogens In Vitro. Phytother. Res. 24: 817–820 

http://onlinelibrary.wiley.com/doi/10.1002/ptr.3014/full


Havsteen BH. (2002).The Biochemistry and medical significance of the flavonoids.Pharmacology & Therapeutics; 96: 67-2002.

http://www.sciencedirect.com/science/article/pii/S016372580200298X



Mehrdad Iranshahi, Ramin Rezaee, Hamideh Parhiz, Ali Roohbakhsh, Fatemeh Soltani (2015) Protective effects of flavonoids against microbes and toxins: The cases of hesperidin and hesperetin Journal of life sciences, 137:125-132.

https://www.ncbi.nlm.nih.gov/pubmed/26188593


Manach C, Scalbert A, Morand C, Remesy C, Jimenez L.(2004) Polyphenols: food sources and bioavailability. American  Journal of Clinical Nutrition ;79:727–47.

http://ajcn.nutrition.org/content/79/5/727.short


Mounnissamy VM, Kavimaini S, Gunasegaran R (2002). Antibacterial activity of gossypetin isolated from Hibiscus sabdariffa, Antiseptic 99(3):81-82.

http://medind.nic.in/imvw/imvw289.html


Olaleye T (2007). Cytotoxicity and antibacterial activity of methanolic extract of Hibiscus sabdariffa. Journal of Medicinal Plants Research, 1(1):9-13.

http://www.academicjournals.org/article/article1380368836_Olaleye.pdf


Ohemeng, K.A., Schwender, C.F., Fu, K.P., Barrett, J.F., (1993). DNA gyrase inhibitory and antibacterial activity of some flavones(1). Bioorg. Med. Chem. Lett. 3, 225–230.

http://www.sciencedirect.com/science/article/pii/S0960894X01808817


Krolicki Z, Lamer-Zarawska E. (1984). Investigation of antifungal effect of flavonoids. Part 1. Herb Pol 30: 53-57.

http://agris.fao.org/agris-search/search.do?recordID=PL8600987


Rauha, J.-P., Remes, S., Heinonen, M., Hopia, A., Kähkönen, M., Kujala, T., Pihlaja, K., Vuorela, H., Vuorela, P., (2000). Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int. J. Food Microbiol. 56, 3–12.

http://www.sciencedirect.com/science/article/pii/S016816050000218X?via%3Dihub


Scalbert A, Williamson G. (2000). Dietary intake and bioavailability of polyphenols. Journal of Nutrition,130:2073S–85S.

http://jn.nutrition.org/content/130/8/2073S.short


Petti S, Scully C (2009). Polyphenols, oral health and disease: A review. J Dent; 37: 413-423.

http://www.sciencedirect.com/science/article/pii/S0300571209000414



Walsh SE, Maillard JY, Russel AD, Catrenich CE, Charbonneau AL, Bartolo RG.(2003). Activity and mechanism of action of selected biocidal agents on Gram -positive and -negative bacteria. J Appllied Microbiology ;94:240–247.

http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.2003.01825.x/full




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